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Developmental strategy included following cloning and fusion steps. 17%) Defensores: 1,431 de 10,207 (14. Firstly, insertion of kanamycin resistance cassette into the suicide vector pEcoR251 was performed. 02%) Total: 3,128 de 14,773 (21. This was followed by ligations of a nocardial replicon from the pCY 104 and EcoRI endonuclease from the pEcoR251, lacking ampicillin resistant determinant.

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